what is your conclusion? . which of the tubes is most hypotonic to the blood cel
ID: 209606 • Letter: W
Question
what is your conclusion?
. which of the tubes is most hypotonic to the blood cels2 . Prepare wet mounts of samples from tubes 1, 4 and 5. Examine under the microscope and re your observations. (Remember to close the iris diaphragm to increase contrast. bservations Tube Solution Time Required for Solution to Become Clear (ransparent) #1 | dH2O #3 #4 #5 0.5% Nad 0.9% Naa |2% NaCl 17 Differentially Permeable Membrane and Simultaneous iffusion and Osmosis erials oaked dialysis tubing or clamps Graduated pipettes and pipette pump Beaker and graduated cylinderExplanation / Answer
Answer to part (a). solution in tube#1 is most hypotonic to blood cells
simply beacuse distilled water is free from any ions or solute ,so it is highly hypotonic and blood whereas have a lot of ions like sodium pottassium and other solutes so it is less hypotonic , and this concentration gradient causes water to enter blood cells which cause these blood cells to rupture and lyse ultimately leaving no blood cell in the solution after two minutes , thus solution becomes clear or transparent after two minutes.
Answer to part (b) when wet mounts will be prepared from these samples in all the tubes , the following observations will/should be made ;
from tube #1- no cells (red blood cells or white blood cells) will be seen as dh20 is highly hypotonic and will lyse the cell by bursting it .
from TUBE#2- 0.1% Nacl is also highly hypotonic but less than dh20 , cells will lyse , no intact cells seen
from tube #3- 0.5% Nacl will be hypotonic but less than 0.1%Nacl , so will again cause lysis but little slow , only few cells seen on wet mount , most cells have lysed , but given much time , all cells will be lysed .
from TUBE#4- 0.9% Nacl is known to be ISOTONIC , so will bring no change in the shape or size of the cells (RBCs) all blood cells seen on wet mount prepared from this sample .
from tube #5 - 2% Nacl will be highly hypertonic in nature and water will moving outside the cell(blood) , causing cell shrinkage . so solution not clear and we will see some shrinked cells in the wet mount .
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