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12. DNA glycosylases 13. DNA photolyases 14. UvrBC endonuclease A. Nuclease invo

ID: 209617 • Letter: 1

Question

12. DNA glycosylases 13. DNA photolyases 14. UvrBC endonuclease A. Nuclease involved in the repair of helix distortion by cutting on both sides (not at the immediate location) of a strand of damaged DNA B. An example of a restriction endonuclease that will not recognize methylated TTAA sequences C. A class of enzymes that excise damaged bases from the sugar backbone D. A class of DNA repair enzymes that absorb radiation and restore modified molecules to their original state E. DNA ligase activity F. An enzyme that removes mismatched DNA from the 3' end of DNA

Explanation / Answer

12. DNA glycosylasis are the enzymes which involved in base excision repair in which damaged bases are removed and replaced in intact DNA sugar phosphate backbone.(12=C)

13. DNA photolyasis are involved in DNA repair and have a conserved protein domain which bind a light harvesting co_factor and repair the damaged DNA by UV hence 13= D.

14. Uvr B and Uvr C are involved in excision repair and make two incison on either side of lesion and repair the DNA. They are found in E.coli, hence 14=A