Laboratory 8: Cell Biology and Conservation Biology: Cell Signaling and Motility
ID: 209838 • Letter: L
Question
Laboratory 8: Cell Biology and Conservation Biology: Cell Signaling and Motility in Symbiodinium
(HELP PLEASE)
Group #
Seawater
DMSO
IWR-1
SKL2001
IWR-1 & SKL2001
8-Bromo cAMP
1. What is auto-fluorescence?
2. Based upon results, which signaling pathways are involved in regulating motility? Do these pathways normally enhance or suppress motility?
3. Based upon results with these four pathways, are we trying to stop or activate the motility of Symbiodinium? Do you think it is possible to chemically further stimulate Symbiodinium motility using any of the four pathways? If so, through what pathway and what mechanism?
FINAL QUESTION BELOW ALSO PLEASE!
1) Approach: Create a summary of the overall approach you use to answer the question being addressed.
Group #
Seawater
DMSO
IWR-1
SKL2001
IWR-1 & SKL2001
8-Bromo cAMP
1 12 0 7 15 6 2 11 2 4 0 11 3 8 1 3 0 9 4 9 0 10 11 8 5 20 0 8 18 16 6 15 0 11 12 10 7 8 17 0 10 20 9 9 16 0 8 12 16 10 14 1 12 15 8 11 13 0 7 8 9 12 16 0 10 10 10Explanation / Answer
1) Autofluorescence is the natural fluorescence that occur in cells. The comman compound that give rise to this fluorescence signal including cyclic ring compound like NADPH, collagen and riboflavin as well as aromatic amino acid such as phenylalanine, tryptophan and tyrosine.
2) motility is regulated by 3 pathway GPCR, RTK/RAS, and Wnt pathway. Here, according to result Wnt pathway and GPCR regulate motility. Wnt is free to bind to frizzled , then dishevelled (DVL) is release, DVL free beta catenin from the APC (adenomatous polyposis coli), axin and GSK-3 beta complex which usually signal to distruct beta catenin but if it is not destroyed then alter the gene expression. iWR-1 disrupted the release of beta catenin so it is not go into the nucleus and affect gene expression. Skl-2001 act as Wnt agonist that upregulate beta catenin regulate transcription by disrupting beta catenin and axin interaction thereby preventing beta catenin phosphorylation and proteosomal degradation without affecting the activity of Gsk-3beta. 8- bromo cAMP is activator of cAMP and resist its degradation from cAMP phosphodiesterase . It enhance GPCR pathway.
This pathways enhance motility normally.
3) Here, we are trying to understand regulation of motility by both stoping and enhance the motility in presence of agonist and antagonist. Yes, if we give agonism of any of the pathway will stimulate motility.
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