Bio12490 Problem set 6 1. You conduct a PCR reaction with two PCR machines in yo

Question

Bio12490 Problem set 6 1. You conduct a PCR reaction with two PCR machines in your lab. At the end of the reactions you realize that both amplifications have failed. For the first machine, you realize that after the first cycle the machine remained at 94 degrees Celsius and failed to go to the next stage of the temperature cycle. For the second machine, you find that the machine does not increase temperature beyond 70 degrees Celsius. In each of the above cases, which part of the PCR cycle was hampered?

Explanation / Answer

1.In the first part of the cycle, the annealing step was hindered as temperature remained at 940C and did not come down to or cooled to 50 -650C so that primers could anneal.In the second step ,since the temperature did not exceed beyond 700C, it means the denaturation step was hindered as it requires a temperature of 950C for denaturation to take place.

2.Since lane 3 in the gel is the only lane that shows to shares one band with each of the other lanes, we can confirm that lane 3 contains the DNA of the father.

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