D Question 8 1 pts The following shows the genotype of a partial diploid bacteri

Question

D Question 8 1 pts The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E.coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash () The possible answers indicate with a "+" or a "-" whether -galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2) second in the presence of lactose. (Assume that glucose is not present in the medium.) Genotype F: I- Oc Z-/FI- Ocz- KEY l+ wild-type repressor I - mutant repressor (unable to bind to the opcrator is - mutant repressor (insensitive to lactosel d type operator

Explanation / Answer

Answer -

Here both I- and Oc are mutated and it becomes constitutive expression. I- it not able to bind operator and Oc operator not allows repressor bind with it. Z- leads to b-galacosidase gene is non functional. So even presence or absence of lactose due to constitutive expression it always express the gene but here gene itself becomes mutation so no expression will take place.

Absence of Lactose : " - "   and presence of Lactose : " - "

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