simple oquays For liter 425 ml 00 ml 1000 ml 0425 Practice 0.224 m \" 225 nm 023

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simple oquays For liter 425 ml 00 ml 1000 ml 0425 Practice 0.224 m " 225 nm 023 L_ 750m Part 1: Microscope Parts The compound microscope is a precision instrument. Treat it with respect. When carrying it, always use two hands, one on the base The microscope consists of a stand (base + neck) on which is mounted the stage (or holding microscope slides) and lenses. The ens that you leok theough is the ocular (paired in binocuar scopes): the lens that loouses on the specimen is 00x) mounted on a revolving nosepiece. The 1 with oil-we wont use the oill immersion objective for this course. Positioning the specimen requires that you tum the mechanical which operate n the x-direction, and the other moves the specimen in the y-drection Focusing on the specimen is achieved by knobs thatl move the stage up and down, so that it is closer or larther from the objective There are two knobs, an outer coarse focus and an inner fine The substage condenser directs light hrough the slide into the objective. An iris diaphragm on the substage condenser contols the amount of light reaching the objective, and aso afects the contrast of the specimen Part 2: Magnification The compound microscope has two sets of lenses; the ocular lens or eye piecel which magnifies an object 10 times its normal size, and the objective lenses located on a revolving noseplece, Retate the nto place Each objective magnication of power witten on €such as 4, 10, 40, 00) This number the power of magntaton tor each of the objective lenses. For total magnification multiply the ocular power (10x) tims he objective lens that is in place. For example, E you have a 10x ocular and a 10x objective, the total magnification is:10x Use this information to fill in the following table ili Objective Lense Total Magnification ow power) high power (01 immersion) Part 3: Using the Compound Light Microscope Atter the instructor explains the proper carying procedures, each student should get out a compound microscope and place it belore them on the bench. The instructor will then go over the procedures for using your scope. You will not need to memorize its pars. Complete the following procedure EVERY TIME you get your microscope out and EvVERY TIME you put it away. Getting Started

Explanation / Answer

Practice:

I think it is 1.2 m instead of 1.2 mm

OR

1.2 mm = ____________ m

I am writing the answer for both the conditions

1.2 m = 1200 mm (1.2 x 1000)

OR

1.2 mm = 0.0012 m (1.2 / 1000)

0.224 m = 224 mm (0.224 x 1000)

225 nm = 0.000225 mm (225/1000000)

0.023 L = 23 ml (0.023 x 1000)

750 ml = 0.75 L (750/1000)

50 ml = 0.05 L (50/1000)

Ocular lens Objective lens Total magnification 10 X 4 (scanning) = 40x 10 X 10 (low power) = 100x 10 X 40 (high power) = 400x 10 x 100 (Oil immersion) = 1000x

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