9. (1.5 pts) If you remove the outer mitochondrial membrane, but leave the inner

Question

9. (1.5 pts) If you remove the outer mitochondrial membrane, but leave the inner membrane intact, what experimental treatment could you do to "force" ATP production? 10. (3pts) Electron micrographs show that mitochondria in heart muscle have higher density of cristae that mitochondria in skin cells. Why do you think this is so? 11. (3pts) Mitochondria in liver cells appear to move freely in the cytosol, whereas those in cardiac muscle are immobilized at positions between myofibrils. Why do you think this arrangement might be a functionally important difference between these two cells?

Explanation / Answer

Question 9 answered

ATP production happens at the inner membrane towards the lumen by the proton motive force generated by the gradient of Hydrogen ions across the inner membrane (High on outside i.e. intermembrane space and low in the lumen). SO if we experimentally supply the system with hydrogen ions we can force ATP production.

Q10 answered

The heart must require a huge amount of ATPs so more ATP pumps are required which are located in Cristae so there are more of it in the heart muscle cells.

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