34. Joshua Lederberg and Edward Tatum discovered a sex-like process in bacteria

Question

34. Joshua Lederberg and Edward Tatum discovered a sex-like process in bacteria using A) high-powered microscopy in combination with antibiotic resistant genes B) populations of cells with complementing auxotrophs, and the measurement of the generation of prototrophs during microbial mixing. C) mutations in bacteria that inhibit mating.. D) comparison of bacterial to yeast behavior E) chromosome sequencing and analysis 35. An Ft microbial strain would best be described as: A) any bacterium that readily takes up naked DNA B) incapable of conjugation due to inhibitory genes C) a bacterial strain with specific auxotrophic D) a bacterial strain that carries a phage with E) a bacterium with a plasmid that confers mating through its membrane. on the F plasmid. characteristics. membrane fusion competency competency (or fertility).

Explanation / Answer

34) the answer is B. They used two auxotrops strain of E.coli K12 denoted by strain A and B.A could not synthesise methionine and biotin while B can but not other ( thymine, leucine , threonine which A can).A was grown in the media which contain methionine and biotin B was grown on the medium which contain thy, leu and thr.this expwriment was also called davis tube.This experiment showed that for tranfer of genetic material physical contact is must.

35) a bacterium with a plasmid that confers mating competency ( or fertility).

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