can bec Matching Questions name of the medium with the physiological test. A med

Question

can bec Matching Questions name of the medium with the physiological test. A medium may be used more than once. Tests may require more than one answer a Kligler's iron agar h MR-VP broth cphenol red lactose SIM medium e. Simmon's citrate agar f skim milk agar spinit blue agar h. tryptone broth .3-butanediol fermentation carbohydrate fermentation casein hydrolysis citrate utilization hydrogen sulfide production mixed-acid fermentation triglyceride hydrolysis tryptophan degradation .REAGENTS, Match the name of the reagent with the physiological test. Tests may require more name of the reagent with the physiological test. Tests may require more than one ans wer. a aipha-naphthol h dimethyl-alpha-naphthylamine c. ferric chloride d. Gram's iodine bydrogen peroxide f. Kovac's reagent methyl red L NN.N' N'-tetra i. potassium hydroxide , sulfanilic acid methyl-p-phenylenediamine dihydrochloride 293

Explanation / Answer

Question1. Media

a. MR-VP broth for 2,3-butanediol fermentation.

b. Phenol Red Lactose for Carbohydrate fermentation .

c. Skim milk agar for casein hydrolysis.

d. Klingler's Iron Agar and SIM medium for hydrogen Sulfied production .

e. MR-VP broth for mixed acid fermentation.

f. Spirit Blue agar for triglyceride hydrolysis.

g. Trypton for Tryptophan degradation .

h. Simmon's citrate agar for citrate utilization.

Question 2.Reagents

a. Alpha-naphthol and potassium hydroxide is the reagent of 2,3-butanediol fermentation .

b. Hydrogen peroxide is the reagent of catalase test.

c. Methyl red is the reagent of mixed acid fermentation.

d. Sulfanillic acid and dimethyl -alpha-naphthylamine are the reagent of nitrate reduction .

e.Ferric chloride is the reagent of phenylalanine deamination.

f. Gram's iodine is the reagent of starch hydrolysis.

g. Kovac's reagent is the reagent of tryptophan degradation.

Question 3. Enzymes

a. Protease is the enzyme used in casein hydrolysis.

b. Protease is the enzyme used in gelatin liquefaction.

c. Cysteindesulfurase enzyme used for hydrogen sulfide production.

d. Amylase is used in starch hydrolysis.

e. Lipase is the enzyme used in triglyceride hydrolysis.

f. Urease is the enzyme used for urea hydrolysis.

Question 4. Products

a.Molecular oxygen is the product of catalase.

b. Phenylpyruvic acid is the product of phenylalanine deamination.

c. Fatty acid is the product of triglyceride hydrolysis.

d. Indole is the product of tryptophan degradation.

e. Ammonia is the product of urea hydrolysis.

f. 2,3- butanediol is the product of Voges -Proskauer test.

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