positive Nuaction for Citrate utilization will pwoduce a color cbage a. From dec
ID: 211163 • Letter: P
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positive Nuaction for Citrate utilization will pwoduce a color cbage a. From decp blue to grees & From red to yelow c From colorless to red d From green to deep blue wer True (A) or False (B) to the following questions A bacterial culture grows in Nitrate broth gives a red coloration on addition of Nitrate n This indicates positive reaction for Nitrate oxidation Dammases are cnzymes responsible for dcgradation of proteins Phenol red prodaces a red color in alkaline pll Gas production daring fermentation can be detected using the indicator Methyl red All members of the genus Staphylococci present as cocci in clusters Pseudomonas aenuginosa is positive for Oxidase activity _ lydrogen Peroxide is the reagent of choice for detecting Oxidase activity Streptocoaccus agalactiae is classified Group B beta bemolytic Streptococcus pyogenes is classified as Group A alpha hemolytic Staphylococcus aureus is both mannitol fernenting and coagulase positiveExplanation / Answer
1) True. this test differentiates the bacteria on the basis of their ability to produce nitrate reductase enzyme that hydrolyse nitrate to nitrite which may then again be degraded to various other nitrogen products
The reduction to nitrate to nitrite is tested by addition of sulfanilic acid reagent and alpha naphthylamine. Red colouration indicates positive result
2) true . They remove the maino group from the amino acids
3) true. The colour change seen is yellow to red on pH change to alkaline
4)false. Methyl red though an indicator of mixed acid fermentation, does so by detection of pH change(pH becomes acidic due to production of variety of acids)
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