You are studying a type of yeast that can grow in both the presence and absence
ID: 211921 • Letter: Y
Question
You are studying a type of yeast that can grow in both the presence and absence of oxygen using normal metabolic pathways (glycolysis, Krebs cycle, ETS, ATP synthase). You have generated a mutant that grows well in the presence of oxygen, but not in its absence. You do a stude where you look at the relative levels of NADH in both the mutant and wild-type yeast in the presence and absence of oxygen, and you get the following data:
In no more than 3 sentences, explain why there are normal levels of NADH in the wild-type yeast, but higher levels of NADH in the mutant yeast in the absence of oxygen (white bars)
40 35 30 +Oxygen Oxygen 9 E 25 20 15 10 5 0 Wild-type MutantExplanation / Answer
in the presence of oxygen (aerobic respiration) the level of NADH will decreased. In aerobic respiration, pyruvate is reduced into lactate with the help of NADH. Here NADH reduced into NAD+, hence in the presence of oxygen (aerobic respiration), NADH is reduced so its level is decrease. Vice versa in the absence of oygen the reduction of lacted is decrease and NADH level is increased.
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