5: Using the following plot as a standard, how would Vmax Km, and the graphs loo

Question

5: Using the following plot as a standard, how would Vmax Km, and the graphs look when... Sulutrate ti ite Eneme a)twice as much enzyme is used; b)half as much enzyme is used; c)a competitive inhib is added; d)a pure noncompetitive inhib is added 9: For an enzymatic rxn that obeys M-M kinetics, k-2x108/M-sec, k.1-1x103/sec, and k 5x103/sec, determine Km, kcat? b)Does keat/Km approach the diffusion limit?; c)If the enzyme conc is 2nmol/mL and [S] at saturation, what is Vmax?; d)lf the enzyme conc is 2nmol/mL, what [S] would give v-0.75Vmax2 e)If the enzyme conc is 4nmol/mL and [S] at saturation, what are Vmax and Km?

Explanation / Answer

Ans.(a).

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