We are testing patient’s blood for HIV viral load using the branched chain DNA m

Question

We are testing patient’s blood for HIV viral load using the branched chain DNA method.   We set up standards of 0ng, 1 ng, 5 ng, 10 ng, and 15 ng. After we detect the results, we get densitometry readings for the standards of 0, 5, 10, 15, and 20 respectively. We also ran 3 patient samples and got the following readings: Patient 1= 7, Patient 2=12, and Patient 3=40. What is the concentration of viral DNA in each patient (6 pts) and can we use all of the readings obtained (2 pts)? Patient 1= _____ Patient 2= _____ Patient 3= _____

Explanation / Answer

The Concentration of viral DNA present in each patient is as follows

Patient 1    12

Patient 2    17

Patient 3    45

and we can use all of the readings obtained

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