This is all the information that was given. fill in the the lines. http://www.jb

Question

This is all the information that was given. fill in the the lines.

http://www.jbc.org/content/279/48/50078.full.pdf

D) Antibody _____

     Activated/ inhibited ______________

J) Antibody ________

    Activated/ inhibited _________________

E) Antibody ____

    Activated/ inhibited ________________

K) Antibody _____

    Activated/ inhibited __________________

Based on what we learned from the paper, what would you like to know next? What would be the next step to seeing if this is relevant in humans? (List a few ideas)

_____________________________________

For each of these diagrams in Figure 4, make a note of what antibody is being used, what is being activated and inhibited, and what does this tell you about Tau phosphorylation? MW (kD) MW (KD) e2 80 Forskolin . 100 mMLiCI 80 M Forskolin- 100 M Rp-cAMPS . MW (kD) MW (LD) 2 0.5 0.5 80 JMForskolin 100 mM LicI 80 Forskolin . 100 Rp-CAMPS. MW (kD) + MW (kD)

Explanation / Answer

G) Antibody -PHF-1 (1:500)

inhibited

J) Antibody __PHF-1 (1:500) __

inhibited _______

E) Antibody __Tau-1 (1:30,000)

inhibited ______

K) Antibody ___Tau-1 (1:30,000)

inhibited

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