During DNA replication, ----------- is/are responsible for removal of the RNA-DN

Question

During DNA replication, ----------- is/are responsible for removal of the RNA-DNA hybrid primer

E. primase (in the primasome)

During DNA replication, ----------- is/are responsible for unwinding the DNA double helix and forming the replication bubble

A: DNA polymerase III B. single strand binding proteins C. helicase and gyrase D. DNA polymerase I

E. primase (in the primasome)

During DNA replication, ----------- is/are responsible for unwinding the DNA double helix and forming the replication bubble

A. helicase and gyrase B. DNA polymerase III C. single strand binding proteins D. DNA polymerase I E. primase (in the primasome)

Explanation / Answer

During DNA replication, ----------- is/are responsible for removal of the RNA-DNA hybrid primer

Answer- D. DNA polymerase I

DNA polymerase III carry out DNA synthesis after priming and DNA polymerase I removes the RNA primer by its 5' to 3' exonuclease activity and fill the gaps.

During DNA replication, ----------- is/are responsible for unwinding the DNA double helix and forming the replication bubble.

Answer is - helicase and gyrase.

DNA helicase opens up DNA duplex at replication fork to provide a single stranded template and DNA gyrase stabilizes DNA supercoiling.

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