11. Suppose you spilled two cultures of Salmonella tphinrium (each containing 10

Question

11. Suppose you spilled two cultures of Salmonella tphinrium (each containing 100,000 cells) on your lab bench. You immediately applied the same disinfectant to both cultures at the same time. One culture has been freshly grown for 36 hours and the other culture is two weeks old. If the killing rate of the disinfectant is 90% per minute, do you think that microbes in both cultures will be completely killed after 6 minutes? Why or why not? 12. Three patients in a large hospital acquired infections of Pseudomonas cepacia during their stay. All three patients received cryoprecipitate, which is prepared from blood that has been frozen in a standard plastic blood transfer pack. The transfer pack is then placed in a water bath to thaw. What is the probable origin of the a.

Explanation / Answer

11.

The microbes in both the cultures will probably not kill. This is because the culture which was freshly grown for 36 hours will be in the log phase of growth and might killed with in the six minutes. Log phase is the time of maximum susceptibility to antimicrobial agents. The culture which is two week old will not be in the log phase and will not be susceptible to the same antimicrobial agent. This culture will probably take longer time for all the cells to kill in this culture.

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