Pure Culture Technique(continued 3. Subculture Evaluation With colored pencils,

Question

Pure Culture Technique(continued 3. Subculture Evaluation With colored pencils, sketch the appearance of the growth on the slant dangrams helow. If you and observed Gram-stained slides of the subcultured bacteria, draw a few cells of eac vealed by Gram staining in the adjacent circle. Serratia marcescens for Escherichia coli) Micrococcus luteus (or Chromobacterium violaceum) 4. Compare the results of your streak and pour plates. Which method achieved the best separation of species? 5. Do your slants contain pure cultures? How would you confirm their purities? B. Short-Answer Questions 1. Define the term "colony" as it relates to bacterial growth on solid media. What colony characteristics can be used for differentiation of bacterial species? As the properties of colonies of Serratia marcescens and Micrococcus luteus an exauple, compare on your streak plate. 2. 3. Why is dilution a necessary part of pure culture preparation 90

Explanation / Answer

Ans-4 the streaking method will have clear separation of species because in pour plate method the whol mixture is poured in a space while in streaking it is spread in diffredif region of petridish so they grow according to their streaks.

Ans-5 the purity of the slant culture may be checked by straking it on a petridish and then developing the colonies from the same. If the colonies are of same organism then the culture was pure otherwise not.

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