Examine each strand from the 3\' ---> 5\' direction and locate a DNA triplet of

Question

Examine each strand from the 3' ---> 5' direction and locate a DNA triplet of TAC that will encode for a start codon. Transcribe then translate the DNA nucleotides that follow the start point and translate them into amino acids. Use the the start codon properly to code for Methionine.

3' AATCGATTCCCCGGTAGACG 5'
5' TTAGCTAAGGGGCCATCTGC 3'

What is the amino acid sequence?

Now eliminate the seventh complementary pair of nucleotides from the left hand side of the original double-stranded DNA and reconnect?



Thanks!

Explanation / Answer

3' AATCGATTCCCCGGTAGACG 5'

5' TTAGCTAAGGGGCCATCTGC 3'

on transcription

3'AAUCGAUUCCCCGGUAGACG5' = mRNA (AUG start codon)

amino acid sequence =met-Ala-Ser

met-Ala-Ser-stop

3' AATCGATCCCCGGTAGACG 5' {after removal of 7th pair and connecting)
5' TTAGCTAGGGGCCATCTGC 3'

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