20. (3 points) The following events must occur in order to successful reate a GM

Question

20. (3 points) The following events must occur in order to successful reate a GMO using CRISPR/Cas9. Indicate successfully create a GMO using CRISP R/Cas9. Indicate the order in which these events must occur by labelling them 1-6. Ligase repairs the DNA backbone. One strand of cut genomic DNA base pairs with a homology arm Cas9 binds the sgRNA scaffold sgRNA is expressed from the plasmid. Polymerase uses homology arms and intervening sequence as a template. Cas9 catalyzes a double stranded break.

Explanation / Answer

1. sgRNA is expressed from the plasmid.

2. Cas9 binds the sgRNA scaffold

3. Cas9 catalyzes a double stranded break

4. One strand of cut genomic DNA base pairs with a homology arm

5. Ligase repairs the DNA backbone

6. Polymerase uses homology arms and intervening sequence as a template.

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