QUESTION 6 2 points Save Answe You would like to transcribe a eukaryotic gene in
ID: 214453 • Letter: Q
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QUESTION 6 2 points Save Answe You would like to transcribe a eukaryotic gene in vitro (ie. in a test tube in the lab). Your goal is to achieve a high level of transcription. To your test tube, you add the DNA template for this gene, which contains the following a TATA box sequence located-25 to-30 bp upstream of the transcription start site, the sequence for the gene, and terminator sequences. You also add RNA polymerase and the transcription factors that make up the basal transcriptional machinery. However after doing your experiment, you do not get very high levels of transcription. What do you think might be the cause of this and what corrective measures would you take to increase the level of transcription? T TT Paragraph Arial 3 (12pt) -·- ' Words:0 Path: p Click Save and Subrait to savle and submit. Click Save All Answers to save all answers Close Window Save and Submit Save All Answers US 1 4:36Explanation / Answer
In the above test tube, there is presence of general transcription factors, TATA sequence, transcription site, terminator sequence, RNA polymerase. However, the levels of transcription obtained are not very high. This is because, there is a missing activator sequence in the test tube. Also, along with the activators, there requires to be a co activator sequence. The CO activator is a sequence that would be working along with the transcription factor so as to increase the gene transcription rate.
In this case, we require to add a CO activator sequence along with an activator sequence. The conactivator would be binding to the activator so as to bind to the promoter site. Also, there requires to be a site named as enhancer. The activator and CO activator complex would increase the transcription rate as it would be recruiting the General transcription machinery.
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