Can you help answer these questions? thank you // 1 Aa\"| :: .E-.112 || AaBbCcDe

Question

Can you help answer these questions? thank you

// 1 Aa"| :: .E-.112 || AaBbCcDe AaBbCcto AaBbCi AaBbcc AaBl a a-y. . | | :-. :. L. | | 'Normal 1 No Spaci Heading i Heading 2 Title Paragraph Styles Answer the following questions: Tovisualize your cricket DNA that you isolated in Lab8you will makeard use a 1%agarose gel with a total volume of 50 mls, using TAE as the solvent. Complete the math to determine how much agarose (solid) you need to weigh out to make this gel. Show your calculations. Hint: 1% agarose means 1 gram in every 100 ml 1. 2. What causes DNA fragments to move through the gel during gel electrophoresis? 3. Why do you prepare the gel with TAE and not dH 0? 4. What are the purposes of using DNA loading dye in gel electrophoresis? 6 8 9

Explanation / Answer

A1) to obtain 1% 50mL gel, you would need 0.5g of agarose to be dissolved in 50mL TAE.

This calculation can eaasily be obtained by just dividing 1 by 2 as 100mL divided by 2 is 50mL that is required.

A2) DNA molecules are negatively charged, so when placedd in an electric field like in case of gel electrophoresis, it moves towards the positive plectrode and away from the negative electrode as -ve and -ve repels each other and +ve and -ve attract each other.

A3) TAE is a buffer that is slightly basic that H20 that keeps the DNA deporotonated as well as soluble.

A4) Dna loading dye enables the DNA to get settled on the gel through the TAE buffer. It sort of adds weight to the DNA so that it would not move out of the wells when poured onto the gel.

Feel free to leave a comment down below for any further query. Thank you.

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