In the glycolytic pathway, the enzyme triose phosphate isomerase catalyzes the c

Question

In the glycolytic pathway, the enzyme triose phosphate isomerase catalyzes the conversion of dihydroxyacetone phosphate to glyceraldehyde 3-phosphate, which is then immediately utilized by glyceraldehyde 3-phosphate dehydrogenase to continue through glycolysis. In an experimental cell culture model, inhibition of triose phosphate isomerase leads to cell death under anaerobic conditions. These cells survive, however under aerobic conditions. Please explain these findings. In your answer consider the energy production in aerobic vs anaerobic metabolism. Please be brief.

Explanation / Answer

Triode phosphate isomerise is the 5th enzyme in the glucolytoc It produces 2 products

1.DHAP

2.GAP

So when the TPI(triose phosphate isomerise) enzyme is inhibited it might may be due to different reasons might be congenital in the case Hungary brothers or may be due to feed back mechanisms

DHAP levels increases abruptly it causes to increased levels of AGE products glycosylated products

And as the glycolysis steps cannot move forward

There is no ATP production and hence ATP levels are decreased

And there is decreased oxygen content leading to hypoxia and leading to oxygen stress

As glycolysis is oxygen independent pathway it occurs both in aerobic and anaerobic conditions

But after the inhibition of this enzyme still anaerobic conditions exist there is death of the cell because the other pathways related to DHAP are aerobic in nature

When when oxygen levels are sustained THE other DHAP pathways are activated and the cell may continue its life with avalilability of ATP

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