8. You have two proteins that you suspect cap the ends of actin that end. To det

Question

8. You have two proteins that you suspect cap the ends of actin that end. To determine which protein caps which end, you mix: -free actin monomers at varying concentrations (A) NO ADDED PROTEIN filaments, preventing actin monomers from either adding or leaving at filament an excess of ATP monomer either Protein 1, Protein 2, or neither (control) then let the mixture come to steady state. At that point, you measure the mass of free actin monomers and assembled actin microfilaments0.2 04 0.6 08 in the mixture. The plots on the right summarize the results. actin concentration (B) PROTEIN 1 A. (4pts) From this evidence, please draw a conclusion as to whether Proteins 1 and 2 cap the plus and/or minus ends of actin microfilaments. filament Protein 1 caps which end(s)? - monomer Protein 2 caps which end(s)? - 0 0.2 0.4 0.6 0.8 actin concentration GuM) Q PROTEIN 2 monomer B. (8pts) Please provide the reasoning for your conclusion. filament 0 0.2 0.4 0.6 0.8 actin concentration (M) Figure 16-13 MBo ir The Problems Book (O Garland Sciencr 2

Explanation / Answer

Protein 1 - Prevent actin monomers leaving the pointed end.
Protein 2 - Prevent actin monomers to add on the barbed end.

ATP binds with actin monomers and add to the barbed end of the actin filament. If normal function is happen means filament will increase meanwhile monomers has to decrease shown in the fig.A.

If we inhibit the adding region of filament monomers not able to add on the filaments so, monomers concentration continuously increases. So it leads to decrease in the filament concentration shown in figure C.

If cap formation occurs at the leaving end leads to stop the monomers leaving, so monomers concentration continuously decrease and same time due to continuous addition of monomers in barbed end makes filament concentration increases shown in fig,B.

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