Using the picture above, can you please answer question #2 below with legible ha

Question

Using the picture above, can you please answer question #2 below with legible handwriting. Also, can the explanation very detailed with no confusion.

.ll Airtel 4:00 AM * 91% - Imported File 18-Mar-2018 at 3:52 AM In vitro Methods You are a new doctoral student in the Molecular and Cellular Biology program at Arizona State University. Your first semester of graduate school includes three lab rotations that will help you identify your Ph.D. mentor and research project. In your first rotation, your principal investigator gives you the following components and asks you to use them to demonstrate that a newly-discovered protein is imported into the mitochondrial matrix. [). Cell-free translation system (includes all necessary enzymes and precursors for translating mRNA molecules in a test tube) mRNA for the newly-discovered protein of interest mRNA for a protein known to reside and function within peroxisomes Live rats for isolating and purifying intact mitochondria from rat livers Radioactively-labeled uSmethionine Study the list of components carefully and then answer the questions on the next page. 1. Fill in the boxes below to construct a brief flow chart that demonstrates how the materials provided will be utilized in your experiment. Write the name of each component in an appropriate box. 5 -labeled protein of interest rat liver mitochondria S.labeled peroxisomal protein mport into mitochondria 4 no import into mitochondria

Explanation / Answer

Here in this experiment,we are dealing with newly discovered protein pathway.Proteins are synthesized from amino acids by the process called translation.We are using S35 labelled methionine amino acid which will become the part of Newly discovered protein at the time of translation.

We can not use P32 labelling because this can be done only with nucleotides(DNA and RNA) having phosphate groups.

Here we have to study the protein pathway so we need to labelled amino acids which will be the part of protein so any path followed by protein can be tracked with the help of this amino acid

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