Using the picture above, can you please answer question #4 below with legible ha

Question

Using the picture above, can you please answer question #4 below with legible handwriting. Also, can the explanation be very detailed with no confusion?

.ll Airtel 4:00 AM * 91% - Imported File 18-Mar-2018 at 3:52 AM In vitro Methods You are a new doctoral student in the Molecular and Cellular Biology program at Arizona State University. Your first semester of graduate school includes three lab rotations that will help you identify your Ph.D. mentor and research project. In your first rotation, your principal investigator gives you the following components and asks you to use them to demonstrate that a newly-discovered protein is imported into the mitochondrial matrix. [). Cell-free translation system (includes all necessary enzymes and precursors for translating mRNA molecules in a test tube) mRNA for the newly-discovered protein of interest mRNA for a protein known to reside and function within peroxisomes Live rats for isolating and purifying intact mitochondria from rat livers Radioactively-labeled uSmethionine Study the list of components carefully and then answer the questions on the next page. 1. Fill in the boxes below to construct a brief flow chart that demonstrates how the materials provided will be utilized in your experiment. Write the name of each component in an appropriate box. 5 -labeled protein of interest rat liver mitochondria S.labeled peroxisomal protein mport into mitochondria 4 no import into mitochondria

Explanation / Answer

When we add radiolabeled sulfer in the medium so, radiolabelled amino acids are produced. These radiolabelled amino acids are incorporated into the protein of interest and takes place in mitochondria.

When radiolabelled protein of interest is present in the mitochondria then its radioactivity is measured. If it shows radioactivity then we can say protein of interest is present. When radioactivity os not obtained from mitochondria then no protein of interest in mitochondria.

Peroxispomal and other protein does not have role in this determination because we clearly go with mitochondrial analysis.

For radioactivity analysis:

By autoradiography

Microscopy

Blotting

Radioactive storage screen
:

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