In vivo Methods You decide that dissecting rats and grinding up their livers isn

Question

In vivo Methods You decide that dissecting rats and grinding up their livers isn't to your liking, so you're excited to learn that your second lab rotation will involve work with living yeast cells. One of your new colleagues discovered recently that some of her fully engineered strains of yeast cells secrete insulin only at room temperature (25 °C). When they are grown at warmer temperatures (35 °C), they stop secreting insulin and grow poorly; some strains won't grow at all at warm temperatures. Your new mentor asks you to study several of these strains to determine the cause(s) of their insulin secretion defects. Propose a hypothesis that explains why your colleague's yeast strains aren't able to secrete insulin. Be sure to include the temperature-dependence of these phenomena in your hypothesis. 5.

Explanation / Answer

Ans- the poosible reason behind this will be based on the hypothesis that the yeast strain used for insulin synthesis is a strain which can withstand only at the optimum temperature ranges from 15-30°C .The strain must be a mutant of normal yeast strain which got some mutation in some gene due to which they can behave normally only at room temperature.

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