Help BIOL105-ONLINE Hw5-S2018 v3 RD (1) _ Edited Collabo InsertTable ChartTextSh

Question

Help BIOL105-ONLINE Hw5-S2018 v3 RD (1) _ Edited Collabo InsertTable ChartTextShape Media Comment yuu number an absurdly low number? 3. Illumina Solexa sequencing a. Name two steps Ilumina Solexa sequencing and explain in one sentence it's purpose are: 1) b. Examine the six clusters (there are millions!) obtained from IlIlumipa Solexa sequencing other components/reagents were present to sequence the clusters of c. Cirele one cluster in the first cycle and follow its amplification on the following cycles. below. The fluorescent modified dideoxy-nucleotides used in the reactions were colored T,G, A and C. and genome fragments Specify the resulting sequence here: 4 972

Explanation / Answer

The first question can be answered looking at the steps of the sequencing and choosing from there. I will choose two that I like and explain briefly:

1) Ligation of adapters: Once the DNA is fragmented in shorter parts (200-600 base pairs), short sequencies of DNA called adaptors are attached to this fragments in order to allow them to bind to a flow cell and also be amplified by PCR.

2)Adding bases: The first sequencing cycle begins by adding four labeled reversible terminators, primers, and DNA polymerase. Each of the terminator bases (A, C, G and T) give off a different colour.

Next, we can answer the c) question: Lets circle the upper left cluster and follow that one.

The first base added was Tymine (in red). Next: Tymine again. Next: cytosine. Next: cytosine.

Sequence would be: TTCC

If we choose the upper right cluster it would be: CCCG

Hope it helps! Good luck

Related Questions

Navigate

• Browse (All)
• Browse H
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.