Hello please help me with this bio question 2. A. You want to use PCR to amplify

Question

Hello please help me with this bio question

2. A. You want to use PCR to amplify the entire DNA shown in the figure below. Of the listed primers, choose the pair that will allow you to amplify this stretch of DNA by PCR. (9 points total) DNA to be amplified: 5’-CGATTCGAGCCATTCGAACTCGATCAGCCGATTCGATCAACCTTGGACAGTCAG-3’ 3’-GCTAAGCTCGGTAAGCTTGAGCTAGTCGGCTAAGCTAGTTGGAACCTGTCAGTC-5’ Primers: (1) 5’-GCTAAGCTCGGTA-3’ (5) 5’-CTTGGACAGTCAG-3’ (2) 5’-GAACCTGTCAGTC-3’ (6) 5’-CGATTCGAGCCAT-3’ (3) 5’-CTGACTGTCCAAG-3’ (7) 5’-ATGGCTCGAATCG-3’ (4) 5’-GACTGACAGGTTC-3’ (8) 5’-TACCGAGCTTAGC-3’ Answer: I will need primer number _____ and primer number _____. (3 points each) B. How many PCR cycles do you need to perform in order to obtain 128 double-stranded copies of a DNA molecule, if you start with a single molecule of double-stranded DNA and assume that your PCR steps are 100% efficient? _______________________________ (3 points)

Explanation / Answer

Forward primer would be primer number 6 and reverse primer would be primer number 3.

If we start with with 1 copy and assume 100 percent efficiency then it would take 2n cycles to make 128 copies.

2n = 128 = 7 cycles

Related Questions

Navigate

• Browse (All)
• Browse H
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.