hi please help with this question 8. Find the most appropriate technique from th

Question

hi please help with this question

8. Find the most appropriate technique from the ones listed on the right for the experimental tasks

listed on the left. Each technique may be used only once. (3 points each; total = 18)

Determining gene’s location on a chromosome _____    A. Use of reporter genes

Production of recombinant protein in bacteria _____    B. Use of expression vectors

Detection of rare viruses in blood _____                           C. Microarrays

Comparison of gene expression profiles _____                  D. In situ hybridization

Analysis of gene regulatory sequences _____                  E. GFP-protein fusions

Determining protein’s subcellular localization _____       F. Polymerase chain reaction (PCR)

Explanation / Answer

Determining gene’s location on a chromosome : D. In situ hybridization

Production of recombinant protein in bacteria : B. Use of expression vectors

Detection of rare viruses in blood : F. Polymerase chain reaction (PCR)

Comparison of gene expression profiles : C. Microarrays

Analysis of gene regulatory sequences : A. Use of reporter genes

Determining protein’s subcellular localization : E. GFP- protein fusions

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