Academic Integrity: tutoring, explanations, and feedback — we don’t complete graded work or submit on a student’s behalf.

on Completion Status: ing to the next question prevents changes to this answer Q

ID: 216763 • Letter: O

Question

on Completion Status: ing to the next question prevents changes to this answer Question 3 of 6 1 points Save Answer the substance needed to its function in the DNA isolation and PCR reaction: DNA polymerase DNA template magnesium ion nucleotides (dNTPs - A, C, G and T) A indicate where on the DNA template the copying process is supposed to begin B provide the DNA sequence to be amplified c provkse the optimum lonic and pH environment D assemble the nucleotides into the complementary DNA sequence E provide a cofactor for the enzyme DNA polymerase oligonucleotide primers salt buffer entary DNA sequence

Explanation / Answer

DNA polymerase- assemble the nucleotides into the complementary DNA sequence

DNA template- provide the DNA sequence to be amplified.

Magnesium ion- provide a cofactor for the enzyme DNA polymerase

Nucleotides- provide the raw materials to assemble the complementary DNA sequence

Oligonucleotide primers- indicate where on the DNA template the copying process is supposed to begin.

Salt buffer- provide the optimum ionic and pH environment.