C 6. (1.5 pts) What would be your prediction if isolated mitochondria were incub
ID: 216774 • Letter: C
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6. (1.5 pts) What would be your prediction if isolated mitochondria were incubated in a medium with excess NADH, but without O2? Choose any answers that apply. a. The intermembrane space would be more acidic than the matrix. b. The intermembrane space would more basic than the matrix. c. The intermembrane space pH will be the same as the matrix. d. ATP synthesis would occur continuously e. ATP synthesis would be reduced or stop altogether f. NADH would NOT be oxidized continuously by the electron transport chain g. NADH would be continuously oxidized by the electron transport chain (1.5 pts) If you treated isolated mitochondria with an excess NADH and O2, but inhibited complex IV using cyanide, what would you predict? Choose any that apply. 7. a. The intermembrane space would be more acidic than the matrix. b. The intermembrane space would more basic than the matrix. The intermembrane space pH will be the same as the matrix. c. d. ATP synthesis would occur continuously e. ATP synthesis would be reduced or stop altogether f. NADH would NOT be oxidized continuously by the electron transport chain g. NADH would be continuously oxidized by the electron transport chainExplanation / Answer
[6. (1.5 pts) What would be your prediction if isolated mitochondria were incubated in a medium with excess NADH, but without O2? Choose any answers that apply.
a. The intermembrane space would be more acidic than the matrix. As more proton will be pumped outside
e. ATP synthesis would be reduced or stop altogether As oxygen is not there.
. NADH would be continuously oxidized by the electron transport chain. As it is present in excess.
(1.5 pts) If you treated isolated mitochondria with an excess NADH and O2, but inhibited complex IV using cyanide, what would you predict? Choose any that apply.
The intermembrane space pH will be the same as the matrix.
e. ATP synthesis would be reduced or stop altogether f. NADH would NOT be oxidized continuously by the electron transport chain
If complex IV can no longer transfer electrons (is blocked), the rest of the electron transport chain will quickly back up and come to a halt. If electrons are no longer moving through the transport chain, protons will no longer be pumped out of the matrix and into the intermembrane space. As the protons already in the intermembrane space flow down their gradient and into the matrix, they won't be replaced, causing the gradient to weaken (and eventually disappear).
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