Undefined/complex media, and give an example Defined media, and give an example
ID: 216834 • Letter: U
Question
Undefined/complex media, and give an example Defined media, and give an example - Selective media . Differential media Nutritional components in media; indicators in media, inhibitors in media Nutrient, Luria, TSB, Thioglycollate Simple, negative, gram stains Aerotolerant, anaerobic, aerobic, obligate(strict) aerobes, facultative anaerobes, aerotolerant anaerobes . . Coliform bacteria * Gram negative, gram positive bacteria Enteric bacteria . Fermentation versus cellular respiration MEDIA CHART TYPE-complex, defined, Purpose selective, differential MEDIA change, or other bacteria Columbia CNA with Sheep Blood Mannitol SaltExplanation / Answer
Columbia CNA with sheep blood
Type: Selective and differential media
Purpose: Used for the isolation as well as differentiation
of gram positive bacteria from clinical and non-clinical
substances.
Result: If there is no growth on the media, then the bacteria is gram
Postive
Individual bacteria response : CNA in media have two antibiotics viz colistin and naladixic acid that can kill gram negative bacteria.
Notes: Ellner in the year 1966 made this media.
Mannitol salt
Type: selective and differential
Purpose: promotes the growth of some specific bacteria whereas inhibits the growth of other bacteria.
Result: if bacteria is able to ferment mannitol, then the agar turns yellow
Individual bacterial response : acidic byproduct that is ferment by bacteria turns media into yellow.
Notes: Commonly , this media is used for isolation of S.aureus and S. epidermidis bacteria.
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