Read the following abstract: \" The aim of the present study was to investigate

Question

Read the following abstract:

" The aim of the present study was to investigate the underlying molecular mechanisms of the potent cell cycle inhibition and apoptotic effect of luteolin on LoVo human colon cancer cells. In the present study, Cell Counting kit-8 assay revealed that luteolin exerted notable cytotoxicity on LoVo cells in a dose- and time-dependent manner, with a 50% inhibitory concentration value of 66.70 and 30.47 µmol/l at the time points of 24 and 72 h, respectively. Flow cytometric analysis confirmed that luteolin promoted cell cycle arrest at the G2/M phase, and subsequently induced cell apoptosis. Western blot analysis further revealed that luteolin exhibited an inhibitory effect on the proliferation of LoVo cells by inhibiting cell cycle arrest at the G2/M phase transition, with an inactivation of cyclin B1/cell division cycle 2 and induction of cell apoptosis, in part via cytochrome c- and deoxyadenosine triphosphate-mediated activation of apoptotic protease activating factor 1. In vivo studies revealed that luteolin effectively decreased the colon tumor body weight of mice. Therefore, the evidence suggests that luteolin may be a potential chemopreventive and chemotherapeutic agent against human colon cancer. "

Write an In-depth question (do not answer it) to the author of this abstract about the subject matter. The question must be open ended.

Explanation / Answer

Please elaborate about the past research conducted in this topic What were your expectations before the start of the experiment. Are your results according to your expectations Please explain what led you to look into the apoptotic activity of luteolin. Please elaborate as to why LoVo cells were the choice of cell line. Have your results been verified by previous works Does previous work results support your findings. What, if anything, could be changed in the procedure Have you faced any hurdles while completing the research. If any, what were they Please elaborate about your expertise and experience level in this particular research Could any other information be added in the paper or the abstract.

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