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ID: 217167 • Letter: P
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proof of the produ proof of the produ proof of the produ proof of the produ 14. A medium such as EMB (Eosin methylene blue) that allows certain bacteria to grow but inhibits medium. others from growing is an example of a a. Complex b. Defined O Selective d. minimal 15. To destroy all forms of life including endospores, all media is sterilized using a. Dry heat b. Radiation exposure for 30 minutes c. A pressure cooker d. Exposure for 15-30 minutes to steam under pressure (15psi) 16. An organism such as????????????is classified as gram negative. a. E coli b. aureus c.P. vulgaris led d. Both a and c are correct 17. Plates should be inverted during incubation to avoid a. Contamination b. Transformation of bacterial cells d. All of the above e. The buildup of condensation on the medium 18. The purpose of heat fixing a smear is to Adhere the cells to the slide b. Preserve their structural integrity c. Kill the cells d. Both a and b are correctExplanation / Answer
14. A medium that allows growth of certain bacteria and inhibit growth of others as called a selective media. This media helps in the selection of resistant variety while inhibiting the growth of non resistants.
15. In order to destroy all forms of life including endospores, we usually boil the sbstance to a prolonged period or heating under high pressure using steam for 30 minutes. Dry heat is not so effective for sterilisation. It requires prolonged exposure to heat.
So the correct answer will be pressure cooker.
16). Both E coli and P vulgaris are gram negative bacteria. S aureus is a gram positive bacteria.
17). Plates are usually inverted during incubation in order to protect it from contamination.
18). Heat fixing a smear increases adherence of bacterial cells to the slide. It also denstures bacterial enzymes thus breaking the cells. The correct option is a.
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