11. What type of staining is most useful for studying the morphology of bacteria
ID: 217420 • Letter: 1
Question
11. What type of staining is most useful for studying the morphology of bacterial cells and characterizing. some of the external structures such as the capsule? a. staining b. staining c. Acid fast staining d. Negative staining 12. Examples of basic dyes used in the Microbiology lab are a. Methylene blue b. Crystal violet c. Safranind. All of the above 13. Two methods available for Microbiologists for obtaining theme a. Dorner method pure cultures are the pour plate method and b. Gram stain c. Streak plate d. Inoculating method 14. A medium such as EMB (Eosin methylene blue) that allows certain bacteria to grow but inhibits others from growing is an example of a medium. a. Complex b, Defined c, Selective d' minimal 15. To destroy all forms of life including endospores, all media is sterilized using a. Dry heat b. Radiation exposure for 30 minutes c. A pressure cooker d. Exposure for 15-30 minutes to steam under pressure (15psi) 16. An organism such as a. E coli is classified as gram negative. d. Both a and c are correct b. S. aureus c. P. vulgaris 17. Plates should be inverted during incubation to avoid a. Contamination b. Transformation of bacterial cells d. All of the above c. The buildup of condensation on the medium 18. The purpose of heat fixing a smear is to a. Adhere the cells to the slide b. Preserve their structural integrity e. Kill the cells d. Both a and b are correct 19. Gram staining is an example of a. Acid fast staining b. Acid staining c. Differential staining d. All of the above 20. Which of the following statement are true regarding gram positive bacteria a. Cell wall has a thick peptidoglycan laver b cell wall hasa s tn an layer d. All of the above are true of gram positive bacteria 21. After ethanol treatment, an organism such as E. coli can be visualized a. Only by counterstaining with crystal violet c. Only by the addition of lodine b. Only by counterstaining with safranin d. All of the above 22. In Gram staining the primary stain used is Methylene blue b. Crystal violetc. Safranin d. Malachite greenExplanation / Answer
11. Option D is correct.
Negative staining is used to study external morphologies of microbes.
12. Option D is correct.
Basic dyes = Crystal violet, safranin, and methylene blue.
13. Option C is correct.
Methods to identify pure cultures = Pour plate method and streak plate method.
14. Option is C correct.
Eosin methylene blue is a selective and differential medium.
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