EDOVO-KIT #301 Study Questions 2. Which of the following pairs could be ligated
ID: 217499 • Letter: E
Question
EDOVO-KIT #301
Study Questions
2. Which of the following pairs could be ligated together? (All termini are cohesive and complementary.)
5'-dephosphorylated linear insert DNA + linear vector
Supercoiled vector + linear insert DNA
5'-dephosphorylated linear vector + linear
5'-dephosphorylated insert DNA
Linear 5'-dephosphorylated vector + linear insert DNA
Nicked vector + linear insert DNA
In general, which of the above possibilities would be the best approach in a subcloning experiment like the one you have done? Why?
Explanation / Answer
Nicked vector + linear insert DNA - will be the best approach in a subcloning experiment.
because other possiblities are not possible due to the presence of 5' dephosphorylated DNA, as 5' phosphate group and 3' hydroxyl group is needed to form the phosphodiester bond.
Linear or nicked form of DNA is more favourable than supercoiled because it lack the free 5' phosphate group for ligation.
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