in a thin 2. Read the DNA sequence from the florescent peaks obtained from elect
ID: 217843 • Letter: I
Question
in a thin 2. Read the DNA sequence from the florescent peaks obtained from electrophoresis capillary tube (label the ends) ATCAGCT 10 Time 3. Which of the following is/are associated with tl A. mRNA B. DNA C. cDNA l)-?N,Pfobe_ E. G. Agarose gel electrophoresis H. Chemiluminescence Process Southern blotting Northern blotting Fluorescence In Situ hybridization Restriction enzymes F DNA-denaturation Component(s) O. F 4. Classify the statements as True or false Statement True/False Mutagenesis cannot be used to study the result of gene mutation Quantitative PCR does not require a primer DNA Hybridization is conducted in a gene micro arra Shortgun is referred to whole genome sequencingExplanation / Answer
5’ ATCAGCT 3’
3’ TAGTCGA 5’
Southern blotting is the transfer of DNA fragments from an electrophoresis gel to a membrane support, resulting in immobilization of the DNA fragments, so the membrane carries a semipermanent reproduction of the banding pattern of the gel. After immobilization, the DNA can be subjected to hybridization analysis, enabling bands with sequence similarity to a labeled probe to be identified.
A northern blot is a laboratory method used to detect specific RNA molecules among a mixture of RNA.
Southern Blotting: DNA, Restriction Digestion, Gel Electrophoresis, Denaturation, Chemiluminescence
Northern Blotting: mRNA, Gel Electrophoresis, Denaturation, Chemiluminescence
Fluorescence In Situ Hybridization: DNA, mRNA, cDNA,, Restriction Digestion, Gel Electrophoresis, Denaturation, Chemiluminescence
Mutation cannot study the result of gene mutation: FALSE
Quantitive PCR does not require a Primer: FALSE
DNA hybridization is conducted in a gene microarray: TRUE
The shotgun is referred to whole genome sequencing: TRUE
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