the nated eye 2. How could you prepare 10 ml of bacteria culture solution of a 1
ID: 217848 • Letter: T
Question
the nated eye 2. How could you prepare 10 ml of bacteria culture solution of a 1/10 dilution of bacterial culture? (2 po How could you make 100 ml of 1/5 dilution of bacteria cell suspension from a 50 ml stock? (2 points) 3. Can a pure culture be prepared from a mixed-broth or mixed-agar slant culture? Explain. (3 points) yes, the comporents from the mived caltue just need be separatd Act by uwing a Streat platt o dluhon Observation of a streak-plate culture shows more growth in Quadrant 4 than Quandrant 3. Explain this observation in own words. (3 points) 4. 3 5. the imaoculana It could be due to human error, no+ stenliing Why is a needle used to isolate individual colonies from a spread plate or streak plate? (3 points)
Explanation / Answer
ANSWER 2) to create the above mentioned dilution of 1/10, take 1ml of your bacerial culture and dissolve it in 9mL of autoclaved PBS or water and you will have 1/10 dilution.
ANSWER 3) To create 1/5 dilution from stock, take 20mL stock bacterial cell suspension and add 80mL of autoclaved PBS or water to it.
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