please help 1. The figure below shows complementation of a deletion of panT (Apa
ID: 217899 • Letter: P
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please help
1. The figure below shows complementation of a deletion of panT (ApanT), which encodes a transcriptional regulator that responds to a small molecule, dog. Expression of a reporter enzyme (GUS) was measured when the panT gene was absent (-) or present +) on a plasmid. In the wild- type background, when the small molecule dog was added at 10 mM, GUS expression increases, whereas GUS expression is low when dog was absent in the culture medium (0 mM), regardless of the presence of panT on the plasmid. In the deletion strain, GUS expression was elevated only when the complementing plasmid carried panT and dog was added to the culture medium. Based on the results in the ApanT mutant and the complementation analysis, what kind of transcriptional regulator does pan T encode? Is expression under positive or negative control? Please explain. (4 points) 1800 1600-panT, 0 mM 1400 2 1200 e 1000 g 800 ??panT, 10 mM ?+ panT, 10 mM O 600 400 200 wild type What kind of transcriptional regulator is PanT? Is PanT-regulated expression under positive or negative control? Explain.Explanation / Answer
1. Pan T is a transcriptional activator. It requires the presence of dog molecule for its activation.
2. Pan T expression/activity is under positive control. When pan T is absent, the dog molecule can not perform any function. Similarly, pan T is non-functional in the absence of the dog molecule. Only when both pan T and dog are present, downstream events can be observed (GUS activity). This data shows that pan T activity is under positive regulation by dog molecule.
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