To test whether these amino acid 4. trieval in the context of the untagged, full

Question

To test whether these amino acid 4. trieval in the context of the untagged, full-length US3 protein, the a identified in Figure 2 were mutated in the full-length US3 protein, the amino s are sntants were then expressed in a modifhied cervical carcinoma cell called HtTA me mare modified Hela cells) and the sub-cellular localization of each mutant was eamined using co-immunofluorescence. PDI US3 Merge GM130 Merge GM130 A. Merge,r Merge GM130 FIG. 4. Essential rok of three amino acid residues for ER retention in the contest of homologous US3. Manine replacement motations were directly introdaced into the region proteins was analyzed by comfocal laser mctoscopy. HeTa cels stably exprosing wild-type US3 orA or mutants were fised, permeabilized, and double innnusostained with ana-US3 for each mutant (midde column and with anti-PDI or asi-GM10 anibody for endogenous marker proteins (left column). The right column shows merged images us US3, as was done for ? 128CiFP. Colocaliation of the U53 mutants with marker GM130 (Golgi matrix protein) expression in this experiment? a. These proteins are directly involved in CMV infection b. These proteins are involved in trafficking of US3 c. These proteins process US3 d. These proteins are markers for different cellular compartments. e. These proteins are markers for CMV infection. 10. (1pt) What is the purpose of looking at PDI (protein disulfide isomerase) and

Explanation / Answer

Answer is d.

PDI and GM130 are Endoplasmic reticulum and Golgi aparatus resident protein respectively. Here they are using them as biomarker for ER and Golgi aparatus.

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