1. You have discovered a novel sigma factor in coli cells called ?25 Based on da

Question

1. You have discovered a novel sigma factor in coli cells called ?25 Based on data from a DNase foot-printing assay you have determined that ?25 binds to consensus sequences at positions 15 and -30 upstream of genes involved in the stress response to lambda bacteriophage infections, including gene z. You hypothesize that in order for gene z to be transcribed the ?25 protein must bind to the -15 and -30 regions. What approach could you use to test this hypothesis? You have already cloned the promoter region (containing the -15 and -30 regions) and the coding region for gene z into pUC19. What molecular techniques could you use to test your hypothesis? What results would you need to get to support your hypothesis? (4 points) dependent termination (2 points). 3. Given the wobble base pairing, only 9 tRNAs are needed to encode the 4 amino acids Ser, Pro, Trp, and Leu. List the mRNA codons for the 4 amino acids and necessary 9 tRNA anti-codons. (4 points) 4. What is the amino acid sequence of the peptide encoded by the following mRNA sequence? Which positions in the mRNA can tolerate a mutation without resulting in a change in amino acid sequence? What changes are tolerated at each of these positions? (2 points) 5-AUGACCAAAUGGGCG-3'

Explanation / Answer

Ans-1 the hypothesis can be tested by observing the binding of o25 protein with the coding region.operon method will be used for this purpose. If the protein will bind to -15 to -30 region then the gene z will be transcribed and lead to production of a specific protein which can be checked by using specific antibody. If o25 will not bind to specific region then there will be no protein production.

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