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Question
Using this article:
https://learn.unt.edu/bbcswebdav/pid-5352245-dt-content-rid-119555542_1/courses/BIOC.3622.508-NT752.1181.1/shimomura_lecture.pdf
Answer the following questions:
1. An important difference between Aequorin and GFP is that:
a.
It binds to actin
b.
It is found in large amounts in jellyfish
c.
It has a cofactor
d. It has the fluorescent compound coded within the amino acid sequence
2.
Dr. Shimomura was awarded the Nobel Prize for his work because it has helped advanced biological imaging technology. His research:
Designed synthetic illuminating chemicals based on theories of chemical structural properties
Studied natural chemicals in animals that evolved bioluminescence over billions of years
Was funded because it promised to help cure cancer
Was successful because it didn't take much time in the lab and didn't smell bad
3.
Dr. Shimomura's research could not have been done today because
The jelly fish population is very low
Environmental regulation would not allow it
The nuclear bombs today are way more powerful than in WWII
He would never get a visa to come from a war torn country
Explanation / Answer
Ans: D It has the fluorescent compound coded within the amino acid sequence
Aequorin was first discovered along with green fluorescent protein in 1961 from the same species of jellyfish.In animal aequorin(calcium-activated photoprotein) occurs together with gfp to yield green light under UV irradiation. Although it has been reported aequorin itself emits blue light. Aequorin mainly gets activated by Ca2+ ions.
Although in modern molecular biology techniques GFP is widely used as a luminescent marker.
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