M 1 2 3 4 5 6 7 89 M 5 kb 50 t900 bp 2 kb 375 bp 43 bp 300 b 50 bp 50 1) You are

Question

M 1 2 3 4 5 6 7 89 M 5 kb 50 t900 bp 2 kb 375 bp 43 bp 300 b 50 bp 50 1) You are trying to amplify a product of 475bp. Which lane(s) contain your desired amplicon? 2) Focus on lanes 1-3 only: The desired amplicon has a size of 147bp. What are the bands marked with "#"? What are three things that you could do in order to eliminate these bands? 3) Focus on lanes 4 &7: What are the bands in the regions marked with"@"? 4) Lanes 1, 7, 8, 9 have a band in the well-what is this? Why is there a band in the well?

Explanation / Answer

Because of using the high concentration of template the unutilized DNA remains.

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