FE Scenario Reading: The following DNA strand is both the template strand and a
ID: 218513 • Letter: F
Question
FE Scenario Reading: The following DNA strand is both the template strand and a parental DNA strand: 5' TGG ATG ACG CGC CCC CAG TGA GGG 3 Group Discussion Questions: 1. Which of the DNA strands (template or coding) is composed of the exact same sequence of nucleotides as the mRNA, except has thymine in the DNA in place of uracil? 2. What is the direction (in polarity terms, 3' for example) in which mRNA is translated? 3. Transcribe the template. Be sure to transcribe it in the proper direction. Assume the appropriate promoter sequence is present for RNA polymerase recognition/binding upstream of this sequence and that terminators are present downstream of the sequence, but just not shown in the DNA sequence above. Make sure you label the 5' and 3' polarity of your transcription product. 4. Translate the transcription product exactly as it would be done in the ribosome. Assume the Shine- Dalgarno sequence is present in the mRNA and that the organism uses the normal start codon (AUG) - that doesn't necessarily mean that its present, however, so if there is not a start codon present in the sequence, there are significant impacts to the answer of this question. When looking for the start codon, don't forget that there are three potential reading frames per codon. If the sequence does not include a stop codon, predict what you expect to occur as a result. Use the codon table from your book or class notes.Explanation / Answer
Q1:- The coding strand or non template strand has the same nucleotide sequence as present in mRNA except that it has thymine (in DNA) instead of uracil in mRNA.
Q2:- The direction of translation of mRNA is 5’ to 3’
Q3:- mRNA 3’ACC UAC UGC GCG GGG GUC ACU CCC 5’
Q4:- polypeptide Thr. Tyr. Cys. Ala. Gly. Val. Thr. Pro
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