S Which of the following vectors hold the largest DNA (in ters of kb) a. Phage c
ID: 218748 • Letter: S
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S Which of the following vectors hold the largest DNA (in ters of kb) a. Phage cloning vectors b. Cosmids C. Bacterial artificial chromosomes d. Yeast artificial chromosomes 16 Which is the best vector for introduce foreign DNA into plant genomesi a. Phage cloning vector b. Ti plasmid of Agrobacterium tumefaciens cosmid d. electroporation 17 Which group of enzymes are popularly called molecular "stichers" or "glue b. Igases C. RNA polymerase d. DNA polymerase 18 In the process of cloning eukaryotic DNIA into prokaryotic cells, the role of reverse transcriptaie s to make a. double-stranded eukaryotic cDNA from mature mRNA b. mature mRNA from precursor mRNA c. bacterial ONA from eukaryotic DNA d. mRNA from DNA 19 PCR requires all of the following EXCEPT a. primers b. DNA pohymerase C. DNA ligase d. DNA of interest. e deoxyrobinucleotides 20 Arrange the following events in the proper sequence for the bioseparation and production of a 1e Isolation of product and volume reduction 3- Removal of solids 4 Polishing a. 1,2,3,4 b. 3,1,2,4 ?. 4.2.3.1 None of the above 21 Cloning vectors have a. an origin of replication b. a selectable marker d. All of the aboveExplanation / Answer
15) d- Yeast artificial chromosomes hold the largest DNA, i.e upto 1000kb.
16) b- Ti plamid of agrobacterium tumefaciens, is the best vector to introduce foreign DNA into plant genome. This is because, agrobacterium has a very well ability of transferring DNA from itself to plants.
17) b- Ligases, because these arethe enzymes that catalyze the joining of two molecules, accompanied by hydrolysis. eg- DNA ligase that joins the DNA during replication. Hence they are also called as stitchers or glue.
18) a- Reverse transcriptase is an enzyme that catalyzes the formation of double stranded eukaryotic cDNA from the template RNA, in the process of cloning of eukaryotic DNA into prokaryotic cells.
19) e- dexyrobinucleotide. PCR i.e polymerase chain reaction requires, DNA template, DNA primers, DNA polymerase,deoxynucleoside triphosphates, a buffer solution, bivalent and monovalent cations. The DNA ligase is used in certain PCR techniques to produce circular plasmids.
21) d- A cloning vector has an origin of replication, a selectabel marker and also cloning sites which could be multicloning sites or polylinker containing many unique restriction sites.
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