2. After membrane fusion occurs via SNARE proteins (SNAREs), the SNARE complex i
ID: 219425 • Letter: 2
Question
2. After membrane fusion occurs via SNARE proteins (SNAREs), the SNARE complex is separated into individual SNARE proteins by the protein NSF. You are studying a t-SNARE that is essential to exocytosis (the process of releasing the contents of a vacuole to the exterior of the cell). You discover a novel mutation in the t-SNARE that prevents its binding to NSF. (A) What is the direct consequence of this mutation to the SNARE complex post membrane fusion? (B) What is the downstream effect on exocytosis?.Explanation / Answer
Answer A -This mutation leads to, non-dissociation of SNARE complex after membrane fusion. SNARE complex must be dissociated after the exocytosis into v-SNARE & t-SNARE, so that they can undergo another round of exocytosis.
Answer B - There is no effect on first exocytosis. First exocytosis will takes place normally, but further exocytosis, may inhibit due to non availability of SNARE proteins. NSF binding to SNARE complex, causes t-SNARE & v-SNARE to get dissociated but mutation affecting NSF binding to t-SNARE will not allow this dissociation. Therefore further rounds of exocytosis will be inhibited.
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