Calculation of Tocal Magnifications Ocular x Objective -Total Power X magnificat

Question

Calculation of Tocal Magnifications Ocular x Objective -Total Power X magnification) imes_ Intermediate imes High power -times- Brightness Without changing any settings, observe the light intensity at low and high magnification. a. Which magnification has the brightest image? b. Why is there a difference? Look at the bottom of each objective. Which has the largest diameter lens? Which has the smallest? Measuring Diameter of Field "Diameter of field" refers to the distance that can be seen through the microscope. Remove the "e” slide from the microscope. Place a clear plastic ruler across the stage so that the ruler can be viewed horizontally through the microscope. Calculate the diameter of field that can be seern under low power by counting the number of millimeters in the field of view. Estimate this distance to the nearest tenth mm. Convert this number into micrometers to determine the Low Power Diameter of Held in um (LPD). To convert millimeter to micrometer, multiply by 1000 What did you determine the diameter of field to be under low power. a. Use the following formula to calculate the high power diameter of field (HPD). HPD - LPD x low power magnification/high power magnification. b. What is the HPD on your microscope? Depth of Field The vertical distance that remains in focus at one time is called the depth of field

Explanation / Answer

lower power = 10X (ocular) X 4X (objective)= 40 X

intermediate power= 10X (ocular) X 10X (objective)= 100X

Higher power= 10X (ocular) X 40X (objective)= 400X

Which magnification has brighter image?

Low power has brightest image

which has largest diameter lens and which has smallest?

low power objective has largest diameter lens and high power objective has smallest diameter lens.

Diameter of field under low power

using ruler it is around 4.2mm or 4200 micrometre

high power diameter on microscope

HPD= LPDX low power magnification/high power magnification

4.2X 4/400= 0.042mm

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