If you cannot see the order of the numbers on the chart it is: Numbers above lin

Question

If you cannot see the order of the numbers on the chart it is:

Numbers above line from left to right:

1. F27(pointing to the right)

2. F343 or F357(pointing to the right)

3. F515 or F519pointing to the right)

4. F968(pointing to the right)

5. F1177(pointing to the right)

Numbers below the line from left to right:

1. R338(pointing to the left)

2. R534(pointing to the left)

3. R926(pointing to the left)

4. R1492(pointing to the left)

Variable regions from left to right are:

V1, V2, V3, V4, V5, V6, V7, V8, V9

According to the figure below, describe which variable regions will be covered using the given primer sets ( pt each). Cod Primer set A 1492R & F27-Variable Regions Covered: Primer set B F343 & 534R-Variable Regions Covered: Primer set C R1492 & F519-Variable Regions Covered: Why would we choose to use F27 & 1492R as a primer set? Be specific and think about this in terms of the entire molecular process from amplification to identification. (2 points)

Explanation / Answer

Answer:

With set A primer 1492R and F27- variable regions covered are from V1 to V9

With set B primer F343 and 534R - variable regions covered is only V3

With set C primer R1492 and F519- variable regions covered are from V4 to V9

Using the primer set A (1492R and F27) helps in amplification of all the variable regions i.e from V1 to V9. Hence this is advantageous over other primer sets. In addition this primer set amplifies as well as identifies the variable regions present in the genome.

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