TEST (bacterium2) Colony morphology Result Elevation: Growth on superior superfi
ID: 220256 • Letter: T
Question
TEST (bacterium2) Colony morphology Result Elevation: Growth on superior superficial agar exclusively Margin: convex taper Colony shape: Round Colour: White Light passage: Translucent Luster: Shiny Consistency: moist Avg. Diameter: 3 mm Diplobacillus, 4um Positive Cell morphology Gram stain Capsules or Starch Hydrolysis Lipid Hydrolysis H2S reduction Motility Ammonification Litmus milk test Denitrification (NO, to NO,) Ino Denitrification (NO, to NH4 no or Catalase Oxidase Oxygen tolerance Optimal temperature endospores? Capsules No No No Non-motile Some ammonification Reduction of litmus Active no Obligate aerobe 22°C PH 7 Optimal pH | 0.5% Slight growth optimal salt concentration Monnitol salt medium EMB medium TSIA test No Lactose and/or sucrose fermentation Phenylalinine deaminase Gelatinase Antibiotic disk No N30 (Neomycin): ++ Te30 (Tetracyclin):f4 E15 (Erythromycin): + S10 (Streptomycin): +4 C30 (Chloramaphenizol): t1+ t smell growt?Explanation / Answer
1. Lactobacillus spp. (L.plantarum)
2. Bifidobacterium spp. (B.bifidum)
To answer this question one should have a basic knowledge on how the bacteria is classified based on their habitat. Normally the bacteria which causes disease will grow in optimal temperature of 37oC. But here it is mentioned it is 22oC. So it is clearly understood it is not a clinically important bacteria. TSIA test which showed Lactose fermentation tells us it should be a Probiotic bacteria. It is a Gram positive rod and non motile, that narrows us down to Lactobacillus and Bifidobacterium.
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