Locate a journal article where Gel electrophoresis technique is used.Describe th

Question

Locate a journal article where Gel electrophoresis technique is used.Describe the technique briefly based on your study material, and then explain how this technique provided the authors with key information needed for their results and conclusions.Remember to cite and reference the journal article. (25 marks)
Marks will be allocated as follows:
10 marks for a description of the technique, 10 marks for the explanation of how this technique allowed the authors to reach certain conclusions in the article , 5 marks for correct citation and reference.

Explanation / Answer

Gel electrophoresis technique : it is a technique used to separate DNA fragments based on their size and charge. Electrophoresis involves running a current through a gel containing the molecules of interest. Based on their size and charge, the molecules will travel through the gel in different directions or at different speeds, allowing them to be separated from one another.

Explanation:All DNA molecules have the same amount of charge per mass. Because of this, gel electrophoresis of DNA fragments separates them based on size only. Using electrophoresis, we can see how many different DNA fragments are present in a sample and how large they are relative to one another. We can also determine the absolute size of a piece of DNA by examining it next to a standard yardstick made up of DNA fragments of known sizes.gel electrophoresis involves a gel: a slab of Jello-like material. Gels for DNA separation are often made out of a polysaccharide called agrose which comes as dry, powdered flakes. When the agarose is heated in a buffer and allowed to cool, it will form a solid, slightly squishy gel. At the molecular level, the gel is a matrix of agarose molecules that are held together by hydrogen bonds and form tiny pores.At one end, the gel has pocket-like indentations called wells, which are where the DNA samples will be placed Before the DNA samples are added, the gel must be placed in a gel box. One end of the box is hooked to a positive electrode, while the other end is hooked to a negative electrode. The main body of the box, where the gel is placed, is filled with a salt-containing buffer solution that can conduct current. The DNA molecules have a negative charge because of the phosphate groups in their sugar-phosphate backbone, so they start moving through the matrix of the gel towards the positive pole. When the power is turned on and current is passing through the gel, the gel is said to be running.

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